Analyzing Endodontic Infections by Deep Coverage Pyrosequencing
نویسندگان
چکیده
منابع مشابه
Microbial analysis in primary and persistent endodontic infections by using pyrosequencing.
INTRODUCTION The aim of this study was to investigate the bacterial community profile of intracanal microbiota in primary and persistent endodontic infections associated with asymptomatic chronic apical periodontitis by using GS-FLX Titanium pyrosequencing. The null hypothesis was that there is no difference in diversity of overall bacterial community profiles between primary and persistent inf...
متن کاملComparison of Bacterial Community Composition of Primary and Persistent Endodontic Infections Using Pyrosequencing.
INTRODUCTION Elucidating the microbial ecology of endodontic infections (EIs) is a necessary step in developing effective intracanal antimicrobials. The aim of the present study was to investigate the bacterial composition of symptomatic and asymptomatic primary and persistent infections in a Greek population using high-throughput sequencing methods. METHODS 16S amplicon pyrosequencing of 48 ...
متن کاملMicrobiology of Endodontic Infections
Microorganisms play an unequivocal role in infecting root canal system. Endodontic infections are different from the other oral infections in the fact that they occur in an environment which is closed to begin with since the root canal system is an enclosed one, surrounded by hard tissues all around [1,2]. Most of the diseases of dental pulp and periradicular tissues are associated with microor...
متن کاملBiofilms in endodontic infections
Biofilm biology has become an expanding field of research in human, industrial and environmental ecosystems. The knowledge accumulated suggests that organisms growing in biofilms develop properties different to those dwelling in the planktonic state. On surveying the endodontic literature it is obvious that this realization and the fact that biofilms afford the resident microorganisms protectio...
متن کاملComparison of the Bacterial Composition and Structure in Symptomatic and Asymptomatic Endodontic Infections Associated with Root-Filled Teeth Using Pyrosequencing
Residual microorganisms and/or re-infections are a major cause for root canal therapy failure. Understanding of the bacterial content could improve treatment protocols. Fifty samples from 25 symptomatic and 25 asymptomatic previously root-filled teeth were collected from Sudanese patients with periradicular lesions. Amplified 16S rRNA gene (V1-V2) variable regions were subjected to pyrosequenci...
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ژورنال
عنوان ژورنال: Journal of Dental Research
سال: 2010
ISSN: 0022-0345,1544-0591
DOI: 10.1177/0022034510370026